Signal peptide homology between the sweet protein thaumatin II and unrelated cereal α-amylase/trypsin inhibitors
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Signal peptide homology between the sweet protein thaumatin II and unrelated cereal alpha-amylase/trypsin inhibitors.
A cDNA clone (pUP-23) corresponding to a member of a protein family that includes inhibitors of trypsin and of heterologous alpha-amylases has been selected from a library derived from developing barley endosperm and its sequence has been determined. A stretch of 95 nucleotides that included the signal peptide and the first 8 residues of the mature protein was found to be homologous to an exact...
متن کاملCrystal structure of the sweet-tasting protein thaumatin II at 1.27Å.
Thaumatin, an intensely sweet-tasting protein, elicits a sweet taste sensation at 50 nM. Here the X-ray crystallographic structure of one of its variants, thaumatin II, was determined at a resolution of 1.27 Å. Overall structure of thaumatin II is similar to thaumatin I, but a slight shift of the Cα atom of G96 in thaumatin II was observed. Furthermore, the side chain of residue 67 in thaumatin...
متن کاملStructures of three crystal forms of the sweet protein thaumatin.
Three crystal forms of the sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii have been grown. These include two naturally occurring isoforms, A and B, that differ by a single amino acid, and a recombinant form of isoform B expressed in yeast. The crystals are of space groups C2 with a = 117.7, b = 44.9, c = 38.0 A, and beta = 94.0 degrees, P2(1)2(1)2(1) with a = 44...
متن کاملThe relationship between thaumatin, a sweet protein and thaumatopain, a cysteine protease, from the arlis of Thaumatococcus daniellii.
Thaumatin, an intensely sweet protein, is the major proteinaciouscompnentinthe arils ofThaumatococcusdaniellii [l].Thaumatinisamemberofafamilyofatleastfiveisoforms, separable on cation exchange chromatography and all with a molecular weight of approx. 22 kDa and possessing eight disulphide bridges. Thaumatin I and II are derived from distinct genes, the other subforms may result from post-trans...
متن کاملHeterologous Expression of the Secale cereal Thaumatin-Like Protein in Transgenic Canola Plants Enhances Resistance to Stem Rot Disease
Canola (Brassica napus L.) is an important oilseed crop. A serious problem in cultivation of this crop andyield loss, are due to fungal disease stem rot caused by Sclerotinia sclerotiorum. The pathogenesis-related(PR) proteins have the potential for enhancing resistance against fungal pathogen. Thaumatin-like proteins(TLPs) have been shown to have antifungal activity on variou...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1988
ISSN: 0014-5793
DOI: 10.1016/0014-5793(88)80562-3